Imformation about Human Vitamin B6 ELISA Kit

Imformation about Human Vitamin B6 ELISA Kit

Test Principle: The Kit is a solidoid sandwich assay enzyme-linked immunosorbent assay (ELISA). A known  concentration of standard, unknown concentration of sample by adding micro-ELISA-plate for  testing. First incubated it, and biotin-marked antibodies at the same time. After washing,  adding the avidin HRP-marked. After another incubation and washing to remove uncombined enzyme  combo, and then adding substrate A, B, and enzyme combo acted simultaneously. Produce color. The  depth of color and the concentration of samples was proportional relationship.
Kit Performance: 1. Sensitivity: the minimum detection concentration is less than No. 1 standard.       Linear of  dilution. The correlation coefficient R value of linear regression of the sample and  expected  concentration  is 0.990. 
2.Specific: no reaction with other cytokines. 
3. Repeatability: the coefficient of variation of within-plate, inter-plate was less than 10%.